Cloning, Over-Expression, and Purification of ?–Carbonic Anhydrase from an Extremophilic Bacterium: Deinococcus radiodurans

In this study, the cloning, purification and initial characterization of carbonic anhydrase (DrCA) enzyme which we consider to be important in resistance physiology from extremely radioresistant bacteria Deinococcus radiodurans is performed. addition, effect increased gamma irradiation doses on pH-related DrCA activity was determined. after radiation treatment showed that continuously by 6 fold, up first 800 Gy, a decrease observed thereafter. The maximum CO2 hydration for at pH 7.0 40°C. enzyme, homo-dimer complex, slightly thermostable. significantly enhanced several metal ions, especially Zn2+, resulted 5-fold increases activity. Also sulfonamide inhibitory pure enzyme. apparent Km Vmax as substrate were 8.4 mM 637 WAU/mg respectively. assay demonstrated specific purified recombinant enzymes high.